Fig. 1. Effects of AIF regarding the inflammatory responses in LPS-induced RAW264.7 cells. (A) The chemical structure of alpinumisoflavone (AIF). (B) Effect of AIF on cell viability were measured using MTT assay. Levels of (C) NO, (D) TNF-α, (E) IL-6, and (F) IL-1β in culture supernatants were quantitated using ELISA. (G) The protein expression of ICAM-1 were measured using western blot. All values represent mean ± S.D. of three or more replicates. ^###p < 0.001 compared with normal group; *p < 0.05, **p < 0.01 and ***p < 0.001 compared with LPS treated alone group, respectively.