Figure 3.

miR-125b-5p, one of the highly expressed microRNAs (miRNAs) in U-exo, is capable of antiviral action against IFV. A549 cells were transfected with control mimic (Ctl) or miR-125b-5p mimic for 24 hours. The next day, cells were infected with mock or IFV A/H1N1 at an MOI of 1 and RNA was collected 48 h later. (A) The effect of miR-125-5p mimic was verified by quantitative measurement of IL-6R, which is previously shown to be a target of miR-125-5p. (B) Matrix protein 1 (M1), matrix protein 2 (M2) and nucleoprotein (NP) expression levels were quantified and normalized against GAPDH. (C, D) Host gene expressions (retinoic acid-inducible gene-I (RIG-I), melanoma differentiation associated protein 5 (MDA5), mitochondria antiviral signaling protein (MAVS), Stimulator Of Interferon Response (STING), MX Dynamin Like GTPase 1 (Mx1), Interferon stimulated gene 15 (ISG15), O2’-5’-Oligoadenylate Synthetase 1 (OAS1), Interferon Induced Transmembrane Protein 3 (IFITM3), interferon beta (IFN-β), and interferon lambda 1 (IFN-λ1)), were also determined by RT-qPCR (means ± SD; n = 2). *p < 0.05, **p < 0.01, and ***p < 0.001, when compared to Ctl.