Fig. 5. (A) Fluorescence emission spectra of the biosensor in the presence of MUC1 with different concentrations: from bottom to top: 0, 10 pg mL⁻¹, 50 pg mL⁻¹, 100 pg mL⁻¹, 500 pg mL⁻¹, 1 ng mL⁻¹, 5 ng mL⁻¹, 10 ng mL⁻¹, 50 ng mL⁻¹, 100 ng mL⁻¹, 200 ng mL⁻¹ and 300 ng mL⁻¹, respectively. (B) The relationship curve of the fluorescence intensity as a function of MUC1 concentration. While shows the relationship between fluorescence intensity and MUC1 concentration, both experimental conditions: Dnase I, 2 U mL⁻¹; MUC1, 5 ng mL⁻¹; probe, 40 nM; GO, 15 μg mL⁻¹ and the emission wavelength of 519 nm. Error bars: SD, n = 3.