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. 2022 May 9;12:7576. doi: 10.1038/s41598-022-11589-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Profiling the regulatory capacity of genomic sequences using a massively parallel reporter assay. (a) Schematic of MPRA oligonucleotide library design. (b) Schematic of MPRA vector design and cloning. (c) Abundance of MPRA library elements corresponding to genomic sequences represented in the MPRA plasmid pool (pDNA, technical replicates) and expressed in HEK293T (cDNA, biological replicates). (d) Reproducibility of MPRA library element detection across biological replicates. (e) Regulatory activity of reference genome sequences profiled in MPRA (red = significant regulatory activity). (f) Fraction of reference genome sequences that display significant regulatory activity in MPRA. (g) Distance to nearest TSS for reference genome sequences that display significant regulatory activity.