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. 2022 May 9;13:2522. doi: 10.1038/s41467-022-30240-8

Fig. 2. AML causes bacterial dysbiosis, and gut microbiota dysbiosis aggravates the progression of AML.

Fig. 2

a The diversity and richness of the gut microbiota in control mice (without cell injection), control-14days mice (without cell injection after 14 days), AML mice (AML cell injection) and AML-14days (mice after AML cell injection after 14days). Unpaired t-test were used for comparing the Chao1 and Shannon index (n = 5 per group). Data were presented as standard boxplots (with the box encompassing Q1–Q3, the median denoted as a central horizontal line in the box, and the whiskers covering the data within ±1.5 IQR). b Schematic diagram of the mouse experimental process. c Leukaemia cells (GFP+ cells) in the spleen, peripheral blood, and bone marrow from ABX AML mice (n = 5) and control PBS AML mice (n = 5). Details of the gating strategy are described in Supplementary Fig. 11b. d Haematoxylin and eosin–stained histopathology sections and Ki67 immunohistochemical staining of a representative spleen, the ABX AML group, and the PBS AML group. All microscopic analyses were performed at an original magnification of ×80 or ×200, scale bar = 1000 and 275 µm. e Kaplan–Meier survival curve of AML mice (n = 5 per group). f The photographs and weights of spleens from AML-FMT mice (n = 5) and AML-PBS mice (n = 5). g The leukaemia cells (GFP+ cells) in spleen, peripheral blood and bone marrow from AML-FMT mice (n = 5) and AML-PBS mice (n = 5). Details of the gating strategy are described in Supplementary Fig. 11b. h HE histopathology sections of a representative spleen, AML-FMT group and AML-PBS group. All microscopic analyses were performed (original magnification ×80 or ×200), scale bar = 1000 and 275 µm. P values were determined using Dunn’s test in a. P values were determined using unpaired two-tailed t-test in c, f, g. P values were determined using Gehan–Breslow–Wilcoxon test in e. Error bars represent mean ± SEM in a, c, f, g, e. *P = 0.016 Chao1, *P = 0.013 Shannon (a), ***P = 0.0008 PB, **P = 0.0068 SP, **P = 0.0087 BM (c), **P = 0.0043 (e), ***P = 0.0007 (f), **P = 0.0025 PB, **P = 0.0028 SP, *P = 0.0144 BM (g). Source data are provided as a Source Data file.