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. 2022 May 9;12:7583. doi: 10.1038/s41598-022-11596-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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WNT5A enhances the osteo/odontogenic differentiation in hDPSCs. The cells were maintained in osteo/odontogenic medium with either (a–c) recombinant WNT5A or (d–f) WNT2B supplementation. Cells cultured in osteo/odontogenic medium were used as the control. (a and d) Mineral deposition was evaluated at day 14. (b and e) The solubilized staining was measured and quantitated. (c and f) The mRNA levels of osteo/odontogenic marker genes were measured using real-time polymerase chain reaction on day 7. Bars indicate a significant difference between groups (p < 0.05).