Table 5.
Plackett–Burman design matrix where X1, X2…..X9 indicate a number of variables chosen for the study and D1, D2 are dummies for the 12 runs multifactorial design.
| Run No | Pectin | NaNO3 | (NH4)2SO4 | Tryptone | MgSO4 | KH2PO4 | K2HPO4 | CaCl2 | FeSO4 | Dummy | Pectate lyase (μMl/ml/min ) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | D1 | D2 | ||
| 1 | 1.25 | 2.00 | 1 | 2 | 0.3 | 0.05 | 1 | 0.01 | 0.1 | + | − | 0.2210 |
| 2 | 0.25 | 2.00 | 5 | 0.5 | 0.3 | 1 | 1 | 0.01 | 0.1 | − | + | 0.0200 |
| 3 | 1.25 | 0.25 | 5 | 2 | 0.05 | 1 | 1 | 0.25 | 0.1 | − | − | 0.0754 |
| 4 | 0.25 | 2.00 | 1 | 2 | 0.3 | 1 | 0.05 | 0.25 | 0.5 | − | − | 0.0465 |
| 5 | 0.25 | 0.25 | 5 | 0.5 | 0.3 | 0.05 | 1 | 0.25 | 0.5 | + | − | 0.0100 |
| 6 | 0.25 | 0.25 | 1 | 2 | 0.05 | 1 | 1 | 0.01 | 0.5 | + | + | 0.0398 |
| 7 | 1.25 | 0.25 | 1 | 0.5 | 0.3 | 1 | 0.05 | 0.25 | 0.1 | + | + | 0.1432 |
| 8 | 1.25 | 2.00 | 1 | 0.5 | 0.05 | 0.05 | 1 | 0.25 | 0.5 | − | + | 0.1850 |
| 9 | 1.25 | 2.00 | 5 | 0.5 | 0.05 | 1 | 0.05 | 0.01 | 0.5 | + | − | 0.1309 |
| 10 | 0.25 | 2.00 | 5 | 2 | 0.05 | 0.05 | 0.05 | 0.25 | 0.1 | + | + | 0.0333 |
| 11 | 1.25 | 0.25 | 5 | 2 | 0.3 | 0.05 | 0.05 | 0.01 | 0.5 | − | + | 0.1629 |
| 12 | 0.25 | 0.25 | 1 | 0.5 | 0.05 | 0.05 | 0.05 | 0.01 | 0.1 | − | − | 0.0376 |