. 2022 Apr;13(2):695–709. doi: 10.21037/jgo-22-237

Table 1. Oligonucleotide sequences used for the qPCR analysis.

Transcript	Sequence (5'-3' direction)
IL-6R (mouse)	Forward: GCCGGATCCACCTGCCAACCTT
IL-6R (mouse)	Reverse: GGGCCACCGGGAGCAGCAACAC
IL-6R (human)	Forward: GGGGGTAGAGCCGGAAGACAAT
IL-6R (human)	Reverse: CGGCCCGGTAGCATGAATAGTT
STAT3 (mouse)	Forward: CCGGAAGCGAGTGCAGGATCTAG
STAT3 (mouse)	Reverse: CCCGCCAGCTCACTCACAATG
STAT3 (human)	Forward: CACCGGCCGATGCTGGAGGAGA
STAT3 (human)	Reverse: GGGGCCGGTCAGGATGCATGG
GAPDH (mouse)	Forward: TCGTCCCGTAGACAAAATGG
GAPDH (mouse)	Reverse: TTGAGGTCAATGAAGGGGTC
GAPDH (human)	Forward: GGCGCTGAGTACGTCGTGGAGTC
GAPDH (human)	Reverse: CAGCAGAGGGGGCAGAGATGATGA

qPCR, quantitative real-time polymerase chain reaction; IL-6R, interleukin-6 receptor; STAT3, signal transducer and activator of transcription 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.