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. Author manuscript; available in PMC: 2023 May 9.
Published in final edited form as: Annu Rev Biophys. 2021 Dec 21;51:63–77. doi: 10.1146/annurev-biophys-102221-101121

Figure 5.

Figure 5

Thermodynamics of the interaction between AmtB and GlnK. (a) Mass spectrum of purified AmtB–GlnK complex, with the concentration of ADP adjusted to obtain a near equal population of AmtB and AmtB in complex with GlnK. (b) Plot of the natural log of KA (dots) and fit of the non-linear van’t Hoff equation (lines). (c) Native mass spectrometry (MS)–derived thermodynamics of the AmtB–GlnK interaction. The reference temperature is 298 K. (d) Sensorgrams for different concentrations of AmtB injected over a GlnK sensor surface. The fit of a Langmuir 1:1 binding model is shown (grey lines). (e) Surface plasmon resonance (SPR)-derived thermodynamics of the AmtB–GlnK interaction. Figure adapted with permission from Reference 14.