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. 2022 Apr 26;10:824075. doi: 10.3389/fcell.2022.824075

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Copyright © 2022 Abudula, Fan, Zhang, Li, Zhou and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of exosome characteristics. (A) Western blot analysis of exosomal marker CD63, CD81, and CD9 in exosomes from primary cellular supernatant and vaginal secretion. CD9 did not express in exosomes derived from vaginal secretion. (B) Transmission electron microscope (TEM) of exosomes. (C) The average size of exosomes is 95.5 nm. (D). PKH67 staining of exosomes internalization by Normal endometrial stromal cells (NSCs) and HUVECs were observed by the confocal microscope, anti-vimentin was the NSCs marker, HUVECs nuclei labeled with DAPI.