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. 2022 Apr 26;13:868578. doi: 10.3389/fphys.2022.868578

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Copyright © 2022 Kim, Johnson, Peltier, Spitalnik, Hod, Francis, Hudson, Stone, Gordy, Fu, Zimring, Amireault, Buehler, Wilson, D’Alessandro, Shchepinov and Thomas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Free PUFAs in fresh and stored RBCs. Free PUFAs, along with their respective bioactive metabolites (i.e., oxylipins), were measured in fresh and stored blood banks prepared from both C57BL/6J (A) and FVB mice (B). The experiment was repeated twice for FVB mice (2 biological replicates) and each blood bank was analyzed 3 times (3 technical replicates/biological replicate). Breaks in scaling were applied to some of the oxylipin species to allow for graphical visualization of the data for all groups. Groups that do not share common letters are statistically different from one another by one-way ANOVA with Tukey’s multiple comparisons follow-up test (p < 0.05).