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. 2022 Apr 26;10:865787. doi: 10.3389/fbioe.2022.865787

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Copyright © 2022 Liu, Liu, Liu, Zeng, Tian, Long and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cutinase activity of MtCut. (A) MtCut, ICCG, and IsPETase protein purity. The impact of temperature (B) and pH (C) on cutinase activity of MtCut and its mutants. (D) Thermal inactivation profiles of MtCut and its mutants in the absence/presence of 1 mM calcium ions or EDTA at 30°C. All the tests used pNP butyrate as substrate.