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. 2022 Apr 26;10:865787. doi: 10.3389/fbioe.2022.865787

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Copyright © 2022 Liu, Liu, Liu, Zeng, Tian, Long and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural features associated with temperature adaptation. (A) Comparing MtCut and ICCG melting temperature curves. (B) Structures of MtCut and ICCG (PDB entry: 6THT). The catalytic triad (Ser178, Asp224, and His256) is represented as a stick model. Root mean square fluctuations (RMSF) (angstroms) of C_α atoms of MtCut (C) and ICCG (D) structures at different temperatures. The 100 ns simulation trajectories at 35°C and heating from 35 to 70°C for each system were superposed and then used to calculate the RMSF.