FIGURE 3.

Hypo-exos significantly enhance the tube formation of endothelial cells in vitro. (A) Significantly enhanced effect in the Hypo-exos treated group on the formation of network structures by HUVECs shown by tube formation assays (left) and the formed capillary-like structures in two groups were quantitated (right); scale bars = 500 μm. (B) Secretory VEGF levels at 48 h were increased significantly in hypoxic conditioned medium as detected by ELISA. (C) Protein expression levels of MMP-9, ANGPT1 and VEGF were enhanced markedly as shown by western blot analysis (left) and relative levels were qualified as relative fold change to the respective Norm-exos treated cells (right). *p < 0.05, **p < 0.01, ***p < 0.001.