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. 2022 Apr 26;10:879877. doi: 10.3389/fcell.2022.879877

FIGURE 6.

FIGURE 6

Hypo-exos transfer both let-7f-5p and miR-210-3p to promote the tube formation of endothelial cells. (A) Matrigel tube formation assay of HUVECs transfected by mimics, inhibitor and NC of let-7f-5p; scale bars = 500 μm. (B) Matrigel tube formation assay of HUVECs transfected by mimics, inhibitor and NC of miR-210-3p; scale bars = 500 μm. (C) Expression of AGO1 and VEGF validated in endothelial cells transfected by mimics, mimics NC, inhibitor and inhibitor NC of let-7f-5p by qRT-PCR. (D) Expression of EphrinA3 validated in endothelial cells transfected by mimics, mimics NC, inhibitor and inhibitor NC of miR-210-3p by qRT-PC. (E) Expression of EphrinA3 was verified in HUVECs transfected with mimics, mimics NC, inhibitor and inhibitor NC of miR-210-3p by Western blot. (F) Expression of HIF-1a and (G) let-7f-5p and miR-210-3p in SHED cells exposed to hypoxic treatment at 6, 12, 24, and 48 h verified by qRT-PCR. (H) Expression of HIF-1a, nSMase 2 and Rab27a in SHED cells exposed to hypoxic treatment at 6, 12, 24, and 48 h verified by Western blot (left). Quantification of the relative levels of HIF-1a and Rab27a as relative fold change to the respective control cells (right). *p < 0.05, **p < 0.01, ***p < 0.001.