. 2022 May 6;219(6):e20220011. doi: 10.1084/jem.20220011

Table 1.

Cell surface markers defining neutrophil developmental states

Acronym	Full name	Function	Surface markers (mouse)	Surface markers (human)	Overlapping states	References
CMP	Common myeloid progenitors	High proliferation, low self-renewal, multipotent	LKS⁻ CD34^int CD16/32^int Flt3⁺ CD115^lo Antibody combination excludes monocyte-DC progenitors, known as “MDP”	Lin⁻ CD34⁺ CD38⁺ CD45RA⁻		Kwok et al., 2020; Yanez et al., 2017
GMP	Granulocyte–monocyte progenitors	High proliferation, low self-renewal, oligopotent	LKS⁻ CD34⁺ CD16/32^hi Ly6C⁻ Antibody combination excludes committed granulocyte progenitors and monocyte progenitors	Lin⁻ CD34⁺ CD38⁺ CD45RA⁺		Yanez et al., 2017; Kwok et al., 2020
proNeu1	Pro-neutrophils (stage 1)	Committed progenitors; expand in BM during emergency granulopoiesis at the expense of monocytes	LKS⁻ CD34⁺ CD16/32^hi Ly6C⁺ CD115^lo CD81⁺ CD11b⁻ CD106⁻	CD15⁺ CD66b⁺ CD11b⁺ CD49d^hi SSC^lo (also CD34^lo CD38^lo)	Mouse: G0 (Xie et al., 2020); Human: eNeP/N1 (Dinh et al., 2020)	Kwok et al., 2020; Evrard et al., 2018
proNeu2	Pro-neutrophils (stage 2)	Intermediate progeny; do not expand during emergency granulopoiesis	LKS⁻ CD34⁺ CD16/32^hi Ly6C⁺ CD115^lo CD81⁺ CD11b⁺ CD106⁺	CD15⁺ CD66b⁺ CD11b⁺ CD49d^int SSC^hi (also CD34⁻ CD38⁻)	Mouse: G1 (Xie et al., 2020); Human: N1 (Dinh et al., 2020)	Kwok et al., 2020; Evrard et al., 2018
preNeu	Neutrophil precursors	High proliferation, low motility, low effector functions; expand in BM and spleen during emergency granulopoiesis	LCS⁻ cKit^int Ly6C⁺ CD11b⁺ Ly6G^lo CXCR2⁻ CXCR4^hi	CD15⁺ CD66b⁺ CD11b⁺ CD49d^int CD101⁻	Mouse: G2 (Xie et al., 2020), NeuP (Kim et al., 2017), C1/NeP (Zhu et al., 2018); Human: N2 (Dinh et al., 2020)	Kwok et al., 2020; Evrard et al., 2018
immNeu^a	Immature neutrophils	Intermediate proliferation, motility and effector functions	LCS⁻ cKit^lo Ly6C^lo CD11b⁺ Ly6G^int CXCR2⁻ CXCR4^lo	CD15⁺ CD66b⁺ CD11b⁺ CD49d⁻ CD101⁺ CD16^int CD10⁻	Mouse: G3 (Xie et al., 2020), C2 (Zhu et al., 2018); Human: N3 (Dinh et al., 2020)	Kwok et al., 2020; Evrard et al., 2018
mNeu^a	Mature neutrophils	Low proliferation, high motility, high effector functions	LCS⁻ cKit⁻ Ly6C^lo CD11b⁺ Ly6G^hi CXCR2⁺ CXCR4⁻	CD15⁺ CD66b⁺ CD11b⁺ CD49d⁻ CD101⁺ CD16^hi CD10⁺	Mouse: G4 (BM) and G5 (blood; Xie et al., 2020); Human: N4 and 5 (Dinh et al., 2020)	Kwok et al., 2020; Evrard et al., 2018

LKS⁺, Lin⁻ cKit⁺ Sca1⁺; LKS⁻, Lin⁻ cKit⁺ Sca1⁻; LCS⁻, Lin⁻ CD115⁻ SiglecF⁻; Lin, cocktail of lineage marker antibodies, which should include anti-CD11b up to the GMP stage, but exclude it for analysis of proNeu-mNeu.

CXCR2 can be downregulated within tumors; to define mouse immNeu and mNeu in this context, CD101 can be used (CD101⁻ immNeu and CD101⁺ mNeu; Evrard et al., 2018).