Table 4. Overview of studies on detection methods for CTCs in ovarian cancer.
| References | Year | No. of
patients |
Tumor
stage |
Isolation method | Detection method | Targeted antigen/
targeted gene |
Positivity
rate (%) |
HR (95% CI) |
| ND, new disease; RD, recurrent disease; NR, not reported; RT-PCR, real-time polymerase chain reaction; RT-qPCR, real-time quantitative polymerase chain reaction; ICC, immunocytochemistry; BT, before treatment; AT, after treatment; HR, hazard ratio; 95% CI, 95% confidence interval; PFS, progression-free survival; OS, overall survival; NS, not significant; | ||||||||
| Fan,
et al. (26) |
2009 | 66 | I−IV | CAMt (functional
enrichment) |
ICC | EpCAM; CK4, 5, 6, 8, 10, 13, 18 | 60.6 | PFS: 1.44 (0.78−2.64), P=0.040;
OS: 0.89 (0.40−1.95), NS |
| Aktas,
et al. (27) |
2011 | 122 | NR | Immunomagnetic
(Adnatest®) |
RT-PCR
(Adnatest®) |
EpCAM, MUC-1,
CA-125, HER-2 |
19.0 (BT)
27.0 (AT) |
PFS: 1.58 (0.86−2.88), NS
OS: 4.56 (1.94−10.73), P=0.050 |
| Sang,et al.(28) | 2014 | 80 | I−IV | Red blood cells lysis | RT-PCR | MAGE-As | 47.5 | OS: P=0.002 |
| Pearl,
et al. (29) |
2014 | 88 | I−IV | CAMt (functional
enrichment) |
ICC | EpCAM, CA-125,
DPP4 and CKs |
88.6 | PFS: 1.21 (0.49−2.97), P=0.0024;
OS: 1.06 (0.41−2.73), P=0.022 |
| Kuhlmann,
et al. (30) |
2014 | 143 | I−IV | Immunomagnetic
(Adnatest®) |
RT-PCR
(Adnatest®) |
EpCAM, MUC1,
MUC6, ERCC1 |
14.0 | PFS: 1.50 (0.81−2.79), NS
OS: 1.85 (1.03−3.32), P=0.041 |
| Pearl,
et al.(18) |
2015 | 31 | I−IV | CAMt (functional
enrichment) |
ICC/RT-PCR | EpCAM, CA-125, CD44, seprase. Gene expression: EpCAM, CD44, MUC16, FAP | 100 | Risk for PD, P<0.001
OR=121.3 |
| Blassl,
et al.(31) |
2015 | 10 | NR | Immunomagnetic
(Adnatest) |
Multiplex RT-PCR (Adnates) | 19 gene transcripts: epithelial, EMT and stem cell markers | 30.0 | NR |
| Kolostova,
et al. (32) |
2016 | 56 | NR | MetaCell® | ICC/RT-PCR | EpCAM, MUC1, MUC16,
KRT18, KRT19, ERCC1, WT1 |
58.0 | NR |
| Liu,
et al. (33) |
2016 | 10 | NR | Immunomagnetic
(Folic acid) |
ICC | HE4 and FITC AffiniPure | 50.0 | NR |
| Chebouti,
et al. (34) |
2017 | 91 | I−IV | Immunomagnetic
(Adnatest) |
Multiplex RT-PCR (Adnatest) | EpCAM, ERCC1, MUC1,
MUC16, PI3Kα, Akt-2, Twist |
82.0 | I−III:
PFS: 2.35 (1.06−8.74), P=0.042 OS: 7.22 (3.21−111.5), P=0.001 |
| Suh,
et al.(35) |
2017 | 31 | I−IV | Tapered-slit membrane filters | ICC | EpCAM, CK9 | 77.4 | NR |
| Lee,
et al.(36) |
2017 | 24 (ND)
30 (RD) |
I−IV | Microfluidic device | ICC | EpCAM, TROP-2, EGFR, CD45 vimentin, N-cadherin, DAPI | 98.1 | PFS: 1.3 (0.230−7.145), P=0.035
OS: 1.3 (0.350−2.298), NS |
| Rao,
et al.(37) |
2017 | 23 | I−IV | Microfluidics plus immunomagnetic beads (EpCAM) | ICC | EpCAM, CK3–6H5, panCK | 87.0 | NR |
| Obermayr,
et al. (38) |
2017 | 102 (BT)
78 (AT) |
II−IV | Density gradient centrifugation | ICC and FISH | ICC: EpCAM, Cytokeratins,
EGFR, MUC1, HER2 FISH: MECOM, HHLA1 |
26.5 (BT)
7.7 (AT) |
PFS: 5.671 (1.560−20.618),
P=0.008 OS: 3.305 (1.386−7.880), P=0.007 |
| Obermayr,
et al. (39) |
2018 | 20 | II−IV | Microfluidic ParsortixTM | RT-qPCR | EpCAM, PPIC, MAL2,
LAMB1, SERPINE2, TUSC3 |
70.0 | NR |
| Nie,
et al. (25) |
2018 | 20 | NR | Immunomagnetic
(Folic acid) |
ICC | HE4, FA | 80.0 | NR |
| Guo,
et al.(19) |
2018 | 30 | NR | Size-based microfluidic technique | ICC | EpCAM, HE4, panCK, CK7,
Vimentin |
73.3 | NR |
| Po,
et al. (40) |
2018 | 20 | III−IV | Immunomagnetic beads (EpCAM, N-cadherin) | ICC | EpCAM, N-cadherin, CK, Vecad, Vimentin | 90.0 | NR |
| Zhang,
et al. (12) |
2018 | 109 | I−IV | Immunomagnatic beads (EpCAM, HER2, MUC1) | Multiplex RT-PCR | EpCAM, HER2, MUC1, WT1, P16 and PAX8 | 90.0 (BT)
91.0 (AT) |
OS: P=0.041 |
| Kim,
et al.(20) |
2019 | 30 | I−IV | Tapered-slit membrane filters | ICC | CK-9, EpCAM | 76.7 (BT)
57.1 (AT) |
NR |
| Li N,
et al.(41) |
2019 | 30 | I−IV | Immunomagnetic
(EpCAM, FRα) |
ICC | EpCAM, FRα | 67.4 | NR |
| Banys-PM,
et al. (21) |
2020 | 43 | I−IV | Immunomagnetic
(CellSearchTM system) |
ICC (CellSearchTM system) | EpCAM, CK | 26.0 | PFS: P=0.005
OS: P=0.006 |
| Abreu M,
et al. (42) |
2020 | 38
|
I−IV | Immunomagnetic
beads (EpCAM, CK) |
RT-qPCR | GAPDH, TIMP1, CK19, MUC1 | 63.0 | NS |