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. 2022 May 10;28(3):99. doi: 10.1007/s10989-022-10406-0

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© The Author(s), under exclusive licence to Springer Nature B.V. 2022

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Fig. 8 — Evaluation of important parameters for cloning in E. coli. CAI of codon optimized sequence of the vaccine is 1 (A). CAI more than 0.8 is considered appropriate. GC content of the vaccine sequence is 62.42%, which is in the range of 30–60% as appropriate GC content (B). Codon frequency distribution of the vaccine sequence is 100 supporting maximum expression of the vaccine in E. coli (C). Vector map of the vaccine sequence in the pET-28a vector is also illustrated (D)