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. 2022 May 10;18:170. doi: 10.1186/s12917-022-03224-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The mRNA and protein expression levels of Felis catus C11orf96 in different tissues. A-C The dynamic range of qPCR for C11orf96 quantification in the kidney, liver, spleen, lung, intestine, heart, and trachea. The trachea was used as a reference for data analysis. The β-actin acted as an internal reference gene. The trachea served as the analytical reference. All data are obtained from three independent experiments. D The protein expression level in different tissues was determined by western blotting assay. GAPDH was used as the housekeeping gene control. Rabbit polyclonal antibody for C11orf96 was prepared in our laboratory