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. 2022 Apr 26;10:888873. doi: 10.3389/fcell.2022.888873

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Copyright © 2022 Lismont, Revenco, Li, Costa, Lenaerts, Hussein, De Bie, Knoops, Van Veldhoven, Derua and Fransen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Venn diagrams showing the number and overlap of c-IBD-SBP-YAP1C interactors under different experimental conditions. (A) c-DD-DAO-driven H₂O₂ production in the absence or presence of a 24-h chase period. (B) External H₂O₂ addition and elution of the interactors first with 1 M NaCl and subsequently with 10 mM DTT. The threshold values to belong to a specific group reflect the percentage of protein recovered in each fraction relative to the total protein abundance (red: > 66.6% in the NaCl eluate; green: > 66.6% in the DTT after NaCl eluate; brown: less than 66.6% in both eluates).