Peroxisome-derived H2O2 can oxidatively modify multiple glycolytic enzymes. Schematic outlining a simplified overview of glycolysis, the oxidative branch of the pentose phosphate pathway (Ox-PPP), and the glutathione redox cycle. Metabolic enzymes and metabolites are indicated in purple and black, respectively. Enzymes that become sulfenylated upon peroxisomal H2O2 production are shown on a yellow background. Based on evidence found in the literature (Mullarky and Cantley, 2015), oxidation of GAPDH and PKM respectively (i) redirects the glycolytic flux towards ox-PPP (through an increase in the metabolites upstream of GAPDH), and (ii) increases the synthesis of the glutathione precursors glycine and cysteine (through activation of serine synthesis by a buildup of 2PG). 1,3BPG, 1,3-bisphosphoglycerate; 2PG, 2-phosphoglycerate; 3PG, 3-phosphoglycerate; 3PHP, 3-phosphohydroxypyruvate; ALDO, aldolase, CYS, cysteine; DHAP, dihydroxyacetone phosphate; ENO, enolase; F1,6P, fructose-1,6-bisphosphate; F6P, fructose-6-phosphate; G3P, glyceraldehyde-3-phosphate; G6P, ribulose 5-phosphate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GLY, glycine; GPI, glucose-6-phosphate isomerase; GSR, glutathione-disulfide reductase; GSH, glutathione (reduced); GSSG, glutathione (oxidized); HK, hexokinase; NADP+, nicotinamide adenine dinucleotide phosphate (oxidized); NADPH, nicotinamide adenine dinucleotide phosphate (reduced); PFKM, phosphofructokinase, muscle; G6PD, glucose-6-phosphate dehydrogenase; GδL6P, 6-phospho-D-glucono-1,5-lactone; PEP, phosphoenolpyruvate; PGD, 6-phosphogluconate; PGK, phosphoglycerate kinase; PGM, phosphoglucomutase; PHGDH, phosphoglycerate dehydrogenase; PKM, pyruvate kinase; PS, phosphoserine; PSAT, phosphoserine aminotransferase; PSPH, phosphoserine phosphatase; PYR, pyruvate; SER, serine.