Table 1.
Properties of different platforms for the detection of the SARS‐CoV‐2 RNA.
|
Detection methods |
LOD [copies/μL] |
Sample pretreatment and detection time [min] |
Sample volume [μL] |
Purification |
Amplification |
Ref. |
|---|---|---|---|---|---|---|
|
RT‐PCR |
15 |
25/<180 |
10 |
Yes |
Yes |
[7b] |
|
Isothermal ligation |
0.06 |
5/50 |
0.78 |
Yes |
Yes |
[1c] |
|
CRISPR‐Cas12 |
10 |
25/52 |
– |
Yes |
Yes |
[22] |
|
Electrochemical |
6.9 |
60/35 |
2 |
Yes |
Yes |
[15] |
|
FET |
1380 |
35/62 |
10 |
No |
Yes |
[14a] |
|
SPR |
12 |
60/∼10 |
– |
Yes |
Yes |
[23] |
|
Fluorescence polarization |
3 |
25/20 |
2 |
Yes |
Yes |
[24] |
|
Microfluidic biochip |
0.6 |
5/35 |
2–50 |
No |
No |
This work |
Note: The detection time includes the time from sample processing to the reading of the results.