Figure 6:

Image correlation analysis and measuring cytoskeletal flow. a) Analysis on the C. elegans embryo illustrates how PIV can be used to detect protein movement during gastrulation. Images show PIV vectors tracking the movement of myosin clusters (green arrows) and the cell membrane (red arrows). Note that cell membrane movement becomes aligned with centripetal myosin movement at the late stages of gastrulation. Images are taken from (Roh-Johnson et al., 2012). Scale bar: 5μm.

b) STICS analysis performed on a transmitted-light video (left) quantifies the local velocities of yolk granules to measure cytoplasmic activity. Images are taken from (Almonacid et al., 2015). Scale bars: 15μm.