Figure 5. Scanning electron microscope images of KKU-213A cells. Cells were cultured for 10 days in the silk fibroin-gelatin/hyaluronic acid/heparan sulfate (SF-GHHs) 1:2 (A, a), SF-GHHs 1:1 (B, b), SF-GHHs 2:1 (C, c), and silk fibroin (SF) scaffolds (D, d). Distinct spheroids were discerned in SF-GHHs 1:2 compared to other SF-GHHs scaffolds. An elongated cell morphology was observed in the SF scaffold. Scale bars=20 μm, with 600´ magnification (A-D); scale bars=10 μm, with 1,000´ magnification (a-d).
