Skip to main content
. 2022 Apr 26;15:822129. doi: 10.3389/fnmol.2022.822129

Figure 2.

Figure 2

Reduced cerebellar volume and increased apoptosis in Scn8a PC mutant cerebellum. (A) Representative whole-mount images of control and PC Scn8a mutant brains at 9 months of age. The mutant cerebellum appears smaller than control. (B) Magnetic resonance imaging T2 axial and coronal images of the brain showing cerebellar volume loss in the mutant mice at 9–10 months of age (scale bar 10 mm). (C,D) Measurement of the cerebellar area of Scn8a mutant and control mice using T2-weighted 3.0 T MR imaging. Quantitative analysis revealed a significant reduction in cerebellar area in mutant animals in both axial (C) and coronal area (D) (Mean ± SEM; n = 5 per group; **P < 0.01, ***P < 0.001, Student's unpaired t-test). (E,G) Sections of cerebellum of control and PC Scn8a mutant mice at 9–10 months of age labeled with TUNEL showing apoptosis in the molecular cell layer and cerebellar nuclei (Scale bar: 100 μm). (F,H) Percentage of TUNEL-positive cells per unit area of cerebellar molecular layer and deep cerebellar nuclei in the PC Scn8a mice compared with controls (n = 4 for each group, ***P < 0.001, Student's unpaired t-test).