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. 2022 Apr 26;15:822129. doi: 10.3389/fnmol.2022.822129

Figure 3.

Figure 3

Motor coordination impairment and locomotor activity in PC Scn8a mutant mice. (A) Representative walking footprint patterns. (B) Gait footprint analysis at 6 and 12 weeks was evaluated for stride length, fore-base width and hind-base width. Mutant mice displayed ataxic gait with reduced stride length and increased stride width at 12 weeks of age (n > 6 per group. *P < 0.05, **P < 0.01; ***P < 0.001, two-way ANOVA, Bonferroni's post-hoc analysis). (C) Representative traces of mouse activity in an open field during a 15-min recording time. (D) Locomotor activity in the open field was comparable between control (n = 9) and mutant mice (n = 11) in a 15 min time bin (P = 0.4223). Time in minutes is shown on the X-axis. (E) The total distance moved in the open field test was not significantly different between controls and mutants (P > 0.05). (F,G) Mutant mice spent less time (F) in the center of the open field (P = 0.0063), and entered the center of the open field less often (G) than the controls (P < 0.001). Data shown are means ± SEM. ns, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA, Bonferroni's post-hoc analysis for (D). Student unpaired t-test for (E–G).