Figure 5.

Abnormal social behavior in PC Scn8a mutant mice. (A) Representative heat maps showing time spent by Control mice (upper panel) and PC Scn8a mutant mice (lower panel) at each location of the three-chambered apparatus during the test. (B,D) In the social approach test, WT mice spent more time in the chamber with the stranger animal than in the chamber with the inanimate object (B), and spent more time interacting with novel animal (D) in comparison with a novel object, whereas PC Scn8a mutant mice show no preference. (C,E) PC Scn8a mutant mice show no preference for the chamber with the novel animal in comparison with that with a familiar animal in an assay of social novelty (C), and spent similar time interacting with both the novel and familiar animals (E). This is in distinct contrast to control mice. Control: n = 16; Mutants: n = 17, ***P < 0.001, two-way ANOVA, Bonferroni's post-hoc analysis.