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. 2022 May 10;21:111. doi: 10.1186/s12943-022-01549-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — METTL14 regulates mRNA stability of TRAF1. A The mRNA half-life (t1/2) of TRAF1 transcripts in 78S and 78R cell lines. B The mRNA half-life (t1/2) of TRAF1 transcripts in 78S cells with (OEM14) or without (Vec) METTL14 overexpression. C The mRNA half-life (t1/2) of TRAF1 transcripts in 78R cells with (shM14.1 or shM14.2) or without (Scr) METTL14 depletion. D TRAF1 3′-UTR plasmid contain wild-type or mutant seed sequences. E Relative luciferase activity of TRAF1 3′-UTR constructs containing wild-type or mutant seed sequences after co-transfection with vector (Vec), METTL14 overexpression (OEM14) or mutant METTL14 overexpression (M14 Mut) into 78S cells. F Relative luciferase activity of TRAF1 3′-UTR constructs containing wild-type or mutant seed sequences after co-transfection with scramble (Scr) or shMETTL14(shM14) into 78R cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. G qPCR analysis of the mRNA levels of TRAF1 after knock-down of readers. IGF2BP2 was screened out to positively influence the expression of TRAF1. IGF2BP2 was screened out to positively influence the expression of TRAF1. H and I RIP assay for the enrichment of TRAF1 in 78S and 78R incubated with IGF2BP2 antibody. TRAF1 is highly enriched in 78R cells compared to 78S cells. J and K RIP assay for the enrichment of TRAF1 in 78S cells with METTL14 overexpression (J) and in 78R cells with METTL14 depletion(K) incubated with IGF2BP2 antibody. L The decay rate of mRNA and qPCR analysis of TRAF1 at the indicated times after actinomycin D (5 μg/ml) treatment in 78R cells with IGF2BP2 knockdown