Fig. 8.

BCRP3 promotes proliferation and survival in proteotoxic cells. A BrdU incorporation assay for control or BCRP3-deficient HeLa cells treated with 10 µM MG132, 4 µM SB431542 and incubated with BrdU for 24 h. B, C MTT (B), and apoptosis (C) assays for control or BCRP3-deficient HeLa cells treated with 10 µM MG132 for 12 h followed by 4 µM SB431542 for 2 h. Data in (A), (B), and (C) are means ± SD from three independent experiments. P values are determined by two-way ANOVA with Tukey’s post hoc test, **P < 0.01, ***P < 0.001. D, E Western blot analysis of indicated proteins in HeLa cells treated with or without 10 µM MG132 for 16 h, followed by 5 ng/ml TGF-β for 2 h