Fig. 4. Stern–Volmer curves for the fluorescence quenching by cyromazine of different concentrations (0 ppb, 5 ppb, 10 ppb, 50 ppb, 100 ppb and 200 ppb) at four different temperatures (25 °C, 35 °C, 45 °C and 55 °C). The concentration of Tcy2 is 25 nM.