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. 2022 May 10;57(8):1250–1259. doi: 10.1038/s41409-022-01698-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Characterization of HSPCs purified from 100 ng/ml FasL AM treated (a–h) or 400 ng/ml FasL AM treated (i–p) MPBCs and from control MPBCs followed by 7 days expansion. Total cell number per well, (b, j) percent of CD34⁺ cells per total cells in culture. Percentage of HSPCs subpopulations per total CD34⁺ population: (c, k) self-renewal HSCs (CD34⁺CD38^neg/low CD90⁺CD45RA⁻), (d, l) MPPs (CD34⁺CD38^neg/low CD90⁻CD45RA⁻), (e, m) LMPPs (CD38^Neg/LowCD90⁻CD45RA⁺), (f, n) OPPs (CD38^+high) (g, o) early-stage MkPs (h, p) and more mature MkPs (CD38^neg/lowCD41a⁺ and CD38^highCD41a⁺, respectively). N = 3, Mean ± SEM, *P ≤ 0.05 Paired T-test.