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. Author manuscript; available in PMC: 2022 May 10.
Published in final edited form as: J Mater Chem B. 2015 Aug 20;3(36):7271–7280. doi: 10.1039/c5tb00988j

Fig. 7.

Fig. 7

Cellular uptake of polyplexes prepared using either PPDDBP or non-cleavable PDDBP with fluorescein-labeled pDNA was assessed in HCASMCs after 6 h incubation in the presence or the absence of LPS/IFN-γ. (A) Flow cytometry analysis indicates enhanced internalization of LPS /IFN-γ treated PPDDBP/pDNA polyplexes compared to other treatment groups. NT represents the non-treated group. *p < 0.05, where * is in comparison to the other test groups. (B) Confocal images of internalized polyplexes demonstrated distribution of plasmid (green), endosomes (red), and nuclei (blue). The cells were stained with DAPI and endosomal dye Lysotracker to visualize the nucleus and endosomes, respectively. Scale bar: 50 μm. (C) Confocal image analysis of polyplexes supports that LPS/IFN-γ treated PPDDBP/pDNA condition enhance uptake of plasmids significantly compared to all three other conditions. *, o, & indicates p < 0.05, where * is in comparison to LPS/IFN-γ untreated PPDDBP/pDNA, o to LPS/IFN-γ treated PDDBP/pDNA, and & to LPS/IFN-γ treated PPDDBP/pDNA, with N = 10–15 cells in each condition.