Total of 210 samples collected from natural environments of Hawai’i were culture positive for NTM. We detected 80 species, including 26 novel or unnamed species and five species of respiratory relevance, of which M. abscessus and M. porcinum were most common. (A) All samples are shown, with all named species detected at least 2 times shown. The composition of respiratory-relevant species compared to other NTM species is shown based on sample type: water biofilms (B), stream water filters (C), and soil with respiratory-relevant species being more common in water biofilms (D).