FIG 3.

TroABCD promotes the growth of S. suis in Mn-restricted environments. (A) Transcription levels of troA, troB, troC, and troD of S. suis in CDM supplemented with or without Mn. (B) Growth curves of the WT, ΔtroA, ΔtroB, ΔtroC, and ΔtroD strains in CDM without Mn. (C to F) Growth curves of the ΔtroA, ΔtroB, ΔtroC, and ΔtroD strains in CDM with or without metal ions. (G) Intracellular Mn contents of the WT, ΔtroA, ΔtroB, ΔtroC, and ΔtroD strains treated with or without 0.1 mM Mn. The data are expressed as means ± standard deviations from three independent experiments. Asterisks indicate P values of <0.05 by Student’s t tests on the samples. CT, control; OD₆₀₀, optical density at 600 nm.