FIG 5.

PerR played an important role in resistance to H₂O₂ and regulating troABCD. (A) Survival rates of WT and deletion mutant strains treated with 10 mM H₂O₂. (B) Transcription levels of troABCD of S. suis or the ΔperR mutant. (C) Transcription levels of troABCD in the perR mutant treated with or without H₂O₂. (D) An EMSA was performed to evaluate the direct binding of PerR to the promoter region of troABCD. The left panel shows PerR (0 ng, 500 ng, 600 ng, and 700 ng) and a biotin-labeled DNA probe. The right panel shows PerR (700 ng) and a nonspecific DNA probe. The locations of the two probes in the genome are also shown. The data are expressed as means ± standard deviations from three independent experiments. Asterisks indicate P values of <0.05 by Student’s t tests on the samples. ns, not significant.