Fig. 3. Confocal micrographs of HK-2 live cells showing the cy5-labelled guest uptake studies. With the nuclei (blue), plasma membrane (green), cy5 compounds (red), and the merged image visualized. The guests were added to medium at a concentration of 10 μM for a time span of 2 hours, whereafter the cells were washed and visualized by live-cell imaging (the scale bar represents 30 μm).