Fig. 8. EGCG–β-CD complex inhibits osteoclastogenesis-related expression of marker genes and key transcription factor more effectively than EGCG. The RANKL-induced mRNA expression of (A) NFATc1, (B) MMP-9, and (C) TRAP during osteoclastogenesis was determined using qRT-PCR analysis. (D) The expression level of NFATc1 protein was examined by western blotting, and representative images are shown. Gray densities of the bands for NFATc1 were quantified using AlphaView software. Data are presented as the mean ± SEM of three independent experiments. ***p < 0.001 compared with the control; ^###p < 0.001 compared with RANKL treatment only; ^$$$p < 0.001 compared with EGCG treatment.