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. 2022 Mar 31;94(7):3203–3222. doi: 10.1002/jmv.27734

Figure 4.

Figure 4

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) back‐splice junctions (BSJs) are resistant to RNase R treatment. (A) Gel electrophoresis of total RNA with and without RNase R treatment. (B) Northern blotting of host actin mRNA. (C,D) Northern blotting with a BSJ‐spanning N probe (28809–29122 | 28295–28494) under short (C) and long (D) exposure conditions. The size of three circular RNA (circRNA) bands were marked. (E) Reverse‐transcription polymerase chain reaction (RT‐PCR) with convergent primers targeting action and different regions of SARS‐CoV‐2 genome. Divergent primers targeting circHIPK3 was used as positive control for RNase R treatment. (F,G) Inverse RT‐PCR on RNA treated with and without RNase R. Red arrowheads indicate RNase‐R resistant bands.