Skip to main content
. Author manuscript; available in PMC: 2023 Mar 31.
Published in final edited form as: Cell. 2022 Mar 17;185(7):1172–1188.e28. doi: 10.1016/j.cell.2022.02.013

Figure 6. St6 mutant mice are more susceptible to DSS-induced colitis.

Figure 6

(A) Summed intensities of the Neu5Ac and N-glycolylneuraminic acid (Neu5Gc) containing N-glycoforms for total protein and MUC2 in IECs from 12-week-old female WT and St6 mice. FDR = 0.3. NS = not significant. ND = not detected.

(B) Representative confocal micrographs (left) of colon samples stained for cell nuclei (DAPI), mucus (MUC2 antibody), and fluorescence in situ staining for bacteria (EUB) to quantify the thickness of the mucus layer in 10-week-old female WT and St6 mice (right). Scale bar, 100 μm.

(C) Ratio of the quantity of rRNA from infiltrating bacteria isolated from mouse colon mucus of 10-week-old female St6 mice compared to matched WT mice determined by Q-PCR using universal primers for bacterial 16S rRNA genes.

(D-F) Body weight changes (D), representative hematoxylin and eosin staining (E), histological scores (left) and colon length measurements (right) (F) in WT and St6 mice after oral administration at day 10 of 2.5% DSS water. Scale bar, 100 μm.

(G-K) DSS colitis was induced in St6 mice which were treated with either PBS control or mucins. The thickness of the mucus layer was measured before DSS water (G, H) together with analyses of colitis as in (D-F) (I-K). Scale bar, 100 μm. Data represent 3 experiments (A-K). Error bars represent the SD of samples within a group. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

See also Figures S5.