Table 2.

Overview of recent gene therapy by using electroporation techniques

Therapy	Electroporation conditions (Voltage, pulse duration)	Gene	Test model	Reference
Gene expression study	20-50 V, 2.5–5 ms	recombinant Cas9 nuclease and synthetic dual guide RNA	Zebra fish	[114]
Tumor therapy	400 V, 10 ms	pDNA encoding with interleukin -12 (IL-12)	Mouse	[115]
Gene knockdown study	50 V, 25 ms	short hairpin RNA	Sea Anemone	[116]
Vaccination	670 V, 10 ms	Plasmid DNA containing PRRSV antigen	Mouse	[117]
Vaccination	62.5 V, 40 ms	Plasmid DNA containing C179 and S139	Mouse	[118]
Vaccination	70 V, 10 ms 50 V. 10 ms	Cy5-Si RNA	Mouse	[119]
Would healing and vaccination	700 V, 100 μs +  200 V, 400 μs 1600 V,100 μs +	pDNA encoding with hCAP-18/LL37 and ovalbumin	Mouse	[120]
Would healing and tumor treatment	570 V, 100 μs 60 V, 150 ms	pDNA with IL-12, shRNA against endoglin, shRNA	Mouse	[121]
Vaccination	1600 V, 100 μs	pDNA encoding IL-12 with collagen receptor	Mouse	[122]
Vaccination Tumor treatment	700 V, 100 μs 200 V, 400 ms 200 V, 200 ms	pDNA encoding with ovalbumin, luciferase, gp160 against HIV, P1A against P815 mastocytoma	Mouse	[104]