Fig. 2. Effects of GE on the expression of FXR and Nrf2 in vivo and in vitro. (A) GE (150 mg kg−1) induced mRNA expressions of FXR and Nrf2 in ANIT-induced cholestatic mice. (B) Effect of GE (150 mg kg−1) on the protein levels of nuclear FXR, nuclear Nrf2, and cytoplasmic Nrf2 in rats. GAPDH, Lamin B, and β-actin served as the loading control, respectively. (C) Effect of GE on mRNA expressions of FXR and Nrf2 in HepG2 cells. GW4064 (5 μM) and t-BHQ (25 μM) were used as positive control, respectively. (D) Effect of GE (100 μM) on the protein levels of nuclear FXR, nuclear Nrf2, and cytoplasmic Nrf2 in HepG2 cells. Data are expressed as the mean ± S.E.M. (n = 5), *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle or DMSO, #P < 0.05, ##P < 0.01, ###P < 0.001 vs. ANIT group.
