Fig. 3. Effects of FXR and Nrf2 siRNA on the expression of BSEP. HepG2 cells were transfected with either siRNA control (si-Control) or siRNA directed against FXR and Nrf2. Gene silencing efficiencies were measured by (A) western blotting and (B) qRT-PCR. (C) HepG2 cells were treated with either DMSO (0.1%) or GE (100 μM) for 24 h after FXR and Nrf2 knockdown. qRT-PCR analysis was performed to measure the BSEP expression. Results are expressed as mean ± S.E.M. (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001 compared with control siRNA-transfected cells treated with DMSO, ^##P < 0.01 compared with control siRNA-transfected cells treated with GE.