Fig. 4. GE increased FXR and BSEP accumulation in HepG2 cells. The confocal microscopy images of HepG2 cells pre-incubated with DMSO (0.1%) or GE (100 μM) for 24 h. Cells were stained with primary antibodies FXR and BSEP, respectively. Alexa488 was used as the secondary antibody. (A) Nuclear FXR protein and (B) membrane BSEP protein expressions (green) were increased after treatment of GE in HepG2 cells. DAPI staining (blue) was used as a nuclear marker. The bar represents 20 μm.