Fig. 6. Effects of GE on BSEP mRNA levels in CARM1, PRMT1, SRC-1, or PGC-1α-depleted HepG2 cells. HepG2 cells were transfected with either siRNA control (si-Control) or siRNA directed against CARM1, PRMT1, SRC-1, and PGC-1α, respectively. The silencing efficiencies were measured by (A) western blotting and (B) qRT-PCR. (C) BSEP mRNA levels in CARM1, PRMT1, SRC-1, or PGC-1α-depleted cells treated with 100 μM GE are expressed relative to control levels in nontargeting siRNA-treated cells. Data are the mean ± S.E.M. (n = 5). **P < 0.01, ***P < 0.001, compared with control siRNA-transfected cells treated with DMSO, ^##P < 0.01 compared with control siRNA-transfected cells treated with GE.