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. 2018 Nov 5;8(65):37117–37128. doi: 10.1039/c8ra06345a

Primer sequences for qRT-PCR, and oligonucleotide primer for BSEP promoter construct ChIP assay.

Gene name	Direction	Primer sequence 5′ → 3′
Primers for qRT-PCR
hFXR	Forward	TCTCCTGGGTCGCCTGACT
hFXR	Reverse	ACTGCACGTCCCAGATTTCAC
hNrf2	Forward	GCACATCCAGTCAGAAACCA
hNrf2	Reverse	GCCGAAGAAACCTCATTGTC
hBSEP	Forward	ACATGCTTGCGAGGACCTTTA
hBSEP	Reverse	GGAGGTTCGTGCACCAGGTA
hGAPDH	Forward	CTTTGGTATCGTGGAAGGACTC
hGAPDH	Reverse	GTAGAGGCAGGGATGATGTTCT
mFXR	Forward	CTTGATGTGCTACAAAAGCTGTG
mFXR	Reverse	ACTCTCCAAGACATCAGCATCTC
mNrf2	Forward	GCAACTCCAGAAGGAACAGG
mNrf2	Reverse	GGAATGTCTCTGCCAAAAGC
mBSEP	Forward	AGCAGGCTCAGCTGCATGAC
mBSEP	Reverse	AATGGCCCGAGCAATAGCAA
mGAPDH	Forward	CTCCCACTCTTCCACCTTCG
mGAPDH	Reverse	CCACCACCCTGTTGCTGTAG

Primer for ChIP assay
BSEP	Forward	TCTCGTATGTCACTGAACTGT
BSEP	Reverse	ATTTGAGGAAGCCAGAGGAA