Table 1:

Glossary of NMR terms

Chemical shift	Reports on the chemical environment of an atom, and thus can be interpreted with respect to structural state.
Chemical shift perturbation (CSP)	A change in the chemical shift that can report on a variety of factors such as ligand binding or conformational change.
Chemical shift assignments	The assignment of NMR signals to specific amino acids.
Methyl TROSY NMR	An approach used for high molecular weight systems, involves looking at side-chain methyl groups.
ILV labeling	A method for isotropic enrichment of the methyl groups in isoleucine, leucine, and valine side-chains. Used in methyl-TROSY experiments
Uniform labeling	A method for uniform isotopic enrichment of 15N and/or 13C. Traditionally used for lower molecular weight systems.
Paramagnetic enhancement experiment	An experiment that uses a paramagnetic probe to induce relaxation of signals in a distance dependent manner. Can be used to determine the proximity of a paramagnetic-labeled residue within a complex.
Spin relaxation experiments/parameters	A set of experiments/parameters that report on (or can be interpreted with respect to) the amplitude and timescale of residue motions. Under certain conditions these can also report on the chemical shift and population of substates sampled through these motions.
Residue specific rotational correlation times (τC)	Reports on the timescale of motion of residues
Order parameter	Reports on the amplitude of dynamic of residues
15N{1H} heteronuclear NOE (hetNOE)	Reports on the flexibility of residues on timescales less than the overall correlation time.