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. Author manuscript; available in PMC: 2022 Sep 17.
Published in final edited form as: Nat Biomed Eng. 2022 Mar 17;6(3):257–266. doi: 10.1038/s41551-022-00855-9

Figure 1: MAESTRO enables accurate mutation tracking using minimal sequencing in clinical specimens.

Figure 1:

(A) Up to 10,000 MAESTRO probes are designed with stringent length and ΔG for single-nucleotide discrimination of predefined mutations. DNA libraries containing uniquely barcoded top and bottom strands are subject to hybrid capture using allele-specific MAESTRO probes. Only molecules containing tracked mutations are captured and sequenced with duplex consensus for error suppression. (B) Using MAESTRO, the same mutations are discovered using up to 100x less sequencing because uninformative regions are depleted. (C) Probe design overview. See probe design section of Methods.