Figure 3. Activation of NF-κB, MAPK, and NADPH oxidase, and expression of adhesion molecule ICAM-1 were impaired in cxcl1^−/− mice following PMS.

A, CXCL1 is essential for the activation of NF-κB during PMS induction. Phosphorylation of NF-κB/p65(ser 536), and IκBα in the homogenates from liver, lung and spleen kidney from cxcl1^−/− as compared to WT mice. B, Attenuated activation of MAPKs in the organs of cxcl1^−/− mice. Phosphorylated p38-MAPK, ERK, and JNK in the homogenates from different organs from cxcl1^−/− and WT mice were probed with their respective antibodies. C, Expression of adhesion molecule ICAM-1 but not VCAM-1 is reduced in cxcl1^−/− mice. The expression level of ICAM-1 and VCAM-1 were determined in the organs of cxcl1^−/− and WT control mice following PMS for time points 6 and 24 h. D, The activation of NADPH oxidase is impaired in cxcl1^−/− mice. The expression levels of Nox2, p22^phox, p67^phox, and p47^phox were determined in the homogenates of organs of cxcl1^−/− and WT control mice by immunoblotting at 6 and 24 h post-PMS. GAPDH or total p38 expression levels were assessed in all samples as internal loading control and the blots are representative of 2 independent experiments with similar results (A, B, C, and D). (n=4–6/group).