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. 2022 Apr 22;7(8):e157597. doi: 10.1172/jci.insight.157597

Figure 1. mAbs recognized the S2 subunits of both SARS-CoV-1 and SARS-CoV-2.

Figure 1

(A) Multiple sequence alignments of infectious hCoV HR2 regions, including those of SARS-CoV-2, SARS-CoV-1, Middle East respiratory syndrome–related coronavirus (MERS-CoV), and HCoV-OC43, HCoV-HKU1, HCoV-NL63, and HCoV-229E. The CB-119 epitope residues are marked by asterisks. The residues are numbered according to their positions on the SARS-CoV-2 S protein sequence. The red positions represent identical residues, and the black shading indicates highly conserved residues among these sequences. (B) Expression plasmids encoding S proteins from the aforementioned strains were transiently transfected into 293T cells. Subsequently, S protein from each cell lysate was detected by immunoblotting using mAbs. β-actin represents the internal control of each lysate. See complete unedited blots in the supplemental material. (C–E) The binding efficacy of the Mab5 and Mab3-2 mAbs for the (C) recombinant S1 and (D) S2 subunits of SARS-CoV-2 and (E) synthetic peptide CB-119 was determined by antigen-coating ELISA.