Table 2. Number of haplotypes, nucleotide diversity, gene diversity and substitutions identified by gene for the genotyping of the P. aeruginosa strains via MLST.

MLST	H strains (n = 77)	A strains (n = 14)	H + A (n = 91)	acsA	aroE	guaA	mutL	nuoD	ppsA	trpE
Size (nucleotides)	2882	2882	2882	390	498	373	442	366	370	443
Number of Haplotypes	35	14	48	18	12	14	17	9	14	16
Pi	0.0067	0.0074	0.0071	0.0099	0.0102	0.0055	0.0036	0.0024	0.007	0.0101
Eta	0.0057	0.0071	0.0064	0.0066	0.0071	0.0063	0.0049	0.0054	0.0059	0.0084
Hd	0.802	1	0.858	0.769	0.751	0.766	0.678	0.557	0.777	0.779
S	81	65	94	13	18	12	11	10	11	19

H: nosocomial strains; A: environmental strains.

Haplotype was considered as the DNA sequence from the concatenated sequences obtained from the Multilocus Sequence Typing (MLST).

Pi: nucleotide diversity; Eta: theta (per site); Hd: gene diversity; S: substitutions.